Examining F-Actin Interaction with Intact Talin and Talin Head and Tail Fragment using Static and Dynamic Light Scattering
نویسندگان
چکیده
منابع مشابه
Actin flow and talin dynamics govern rigidity sensing in actin-integrin linkage through talin extension.
At cell-substrate adhesion sites, the linkage between actin filaments and integrin is regulated by mechanical stiffness of the substrate. Of potential molecular regulators, the linker proteins talin and vinculin are of particular interest because mechanical extension of talin induces vinculin binding with talin, which reinforces the actin-integrin linkage. For understanding the molecular and bi...
متن کاملPolyphosphoinositides inhibit the interaction of vinculin with actin filaments.
Binding of vinculin to adhesion plaque proteins is restricted by an intramolecular association of vinculin's head and tail regions. Results of previous work suggest that polyphosphoinositides disrupt this interaction and thereby promote binding of vinculin to both talin and actin. However, data presented here show that phosphatidylinositol 4,5-bisphosphate (PI4,5P2) inhibits the interaction of ...
متن کاملAn intramolecular association between the head and tail domains of vinculin modulates talin binding.
Three independent lines of evidence demonstrate an interaction between the head and tail domains of vinculin. First, both the purified 30-kDa tail fragment and a fusion protein containing tail domain, vinculin residues 884-1066, protect purified 95-kDa head domain from cleavage by protease V8 in a specific, dose-dependent manner. Second, direct binding studies between the 95-kDa head and the V8...
متن کاملColocalization of F-actin and talin during Fc receptor-mediated phagocytosis in mouse macrophages
We have studied the distribution of talin in J774 cells and mouse peritoneal macrophages undergoing Fc receptor-mediated phagocytosis. At early stages of phagocytosis, talin accumulates in the cells' cortical cytoplasm adjacent to the forming phagosome and extends into pseudopods that are encircling the particle. Talin colocalizes with F-actin at these sites. After particle ingestion is complet...
متن کاملEntrapment–D-(+)-Glucose Water Nanodroplet: Synthesis and Dynamic Light Scattering
In the present research, the D-(+)-Glucose–confined water nanodroplets with mean size in the range of 6-11 nm were synthesized at D-(+)-Glucose different concentrations by AOT reverse micelles (RMs) method as a function of mass fraction of droplet (MFD) at the constant water-to-surfactant molar ratio ( W=40). The dynamic light scattering of the nano-sized water droplets containing the D-(+)-Glu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1997
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1997.0447a.x